Tootfinder

Progress and new challenges in image-based profiling
Erik Serrano, John Peters, Jesko Wagner, Rebecca E. Graham, Zhenghao Chen, Brian Feng, Gisele Miranda, Alexandr A. Kalinin, Loan Vulliard, Jenna Tomkinson, Cameron Mattson, Michael J. Lippincott, Ziqi Kang, Divya Sitani, Dave Bunten, Srijit Seal, Neil O. Carragher, Anne E. Carpenter, Shantanu Singh, Paula A. Marin Zapata, Juan C. Caicedo, Gregory P. Way

Progress and new challenges in image-based profiling
For over two decades, image-based profiling has revolutionized cellular phenotype analysis. Image-based profiling processes rich, high-throughput, microscopy data into unbiased measurements that reveal phenotypic patterns powerful for drug discovery, functional genomics, and cell state classification. Here, we review the evolving computational landscape of image-based profiling, detailing current procedures, discussing limitations, and highlighting future development directions. Deep learning h…