Tootfinder

Smartphone-integrated RPA-CRISPR-Cas12a Detection System with Microneedle Sampling for Point-of-Care Diagnosis of Potato Late Blight in Early Stage
Jiangnan Zhao (Key Laboratory of Smart Agriculture Systems, Ministry of Education, China Agricultural University, Beijing, PR China, Key Laboratory of Agricultural Information Acquisition Technology, Ministry of Agriculture and Rural Affairs of China, China Agricultural University, Beijing, PR China), Hanbo Xu (Key Laboratory of Smart Agric…

Smartphone-integrated RPA-CRISPR-Cas12a Detection System with Microneedle Sampling for Point-of-Care Diagnosis of Potato Late Blight in Early Stage
Potato late blight, caused by the oomycete pathogen Phytophthora infestans, is one of the most devastating diseases affecting potato crops in the history. Although conventional detection methods of plant diseases such as PCR and LAMP are highly sensitive and specific, they rely on bulky and expensive laboratory equipment and involve complex operations, making them impracticable for point-of care diagnosis in the field. Here in this study, we report a portable RPA-CRISPR based diagnosis system f…

Multimodal Modeling of CRISPR-Cas12 Activity Using Foundation Models and Chromatin Accessibility Data
Azim Dehghani Amirabad, Yanfei Zhang, Artem Moskalev, Sowmya Rajesh, Tommaso Mansi, Shuwei Li, Mangal Prakash, Rui Liao
https://arxiv.org/abs/2506.11182

Multimodal Modeling of CRISPR-Cas12 Activity Using Foundation Models and Chromatin Accessibility Data
Predicting guide RNA (gRNA) activity is critical for effective CRISPR-Cas12 genome editing but remains challenging due to limited data, variation across protospacer adjacent motifs (PAMs-short sequence requirements for Cas binding), and reliance on large-scale training. We investigate whether pre-trained biological foundation model originally trained on transcriptomic data can improve gRNA activity estimation even without domain-specific pre-training. Using embeddings from existing RNA foundati…