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@lpryszcz@genomic.social
2026-03-20 10:06:40

Single-molecule #peptide #sequencing through reverse translation of peptides into DNA
nature.com/articles/s41587…

Fig. 1 | Overview of peptide sequencing through reverse translation. In stage 1, peptides are conjugated with peptide identity barcode sequences immobilized on magnetic beads. A modified Edman degradation reaction is performed using a PITC molecule modified with an azide group, enabling click coupling to a DBCO-modified, biotinylated primer that hybridizes directly to the peptide identity barcode sequence. This primer is extended to record the peptide identity barcode before N-termin…